More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| trefoil factor 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Estrogen Inducible Protein pS2(67-84aa WCFYPNTIDVPPEEECEF). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TFF1 |
Protein | Trefoil factor 1 |
Uniprot ID | P04155 |
Function | Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine. |
Tissue Specificity | Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level). |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 P-type (trefoil) domain. |
Aliases | BCEI antibody|Breast cancer estrogen inducible protein antibody|Breast cancer estrogen inducible sequence antibody|Breast cancer estrogen-inducible protein antibody|D21S21 antibody|Gastrointestinal trefoil protein antibody|Gastrointestinal trefoil protein pS2 antibody|hP1.A antibody|HP1A antibody|HPS 2 antibody|HPS2 antibody|pNR 2 antibody|PNR-2 antibody|pNR2 antibody|Polypeptide P1.A antibody|Protein pS2 antibody|PS 2 antibody|pS2 antibody|pS2 protein antibody|TFF 1 antibody|TFF1 antibody|TFF1_HUMAN antibody|Trefoil factor 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Estrogen Inducible Protein Ps2 antibody, ASA-B0670, IHC(P)
IHC(P): Human Gastric Cancer Tissue
IHC(P): Human Gastric Cancer Tissue
Anti-Estrogen Inducible Protein Ps2 antibody, ASA-B0670, Western blotting
WB: MCF-7 Cell Lysate
WB: MCF-7 Cell Lysate
