More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| mitogen-activated protein kinase 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human ERK1(287-301aa KTKVAWAKLFPKSDS), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAPK3 |
Protein | Mitogen-activated protein kinase 3(MAP kinase 3/MAPK 3) |
Uniprot ID | P27361 |
Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Autophosphorylation at Thr-207 promotes nuclear localization. |
Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. |
Aliases | ERK 1 antibody|ERK antibody|ERK-1 antibody|ERK1 antibody|ERT 2 antibody|ERT2 antibody|Extracellular Signal Regulated Kinase 1 antibody|Extracellular signal related kinase 1 antibody|Extracellular signal-regulated kinase 1 antibody|HGNC6877 antibody|HS44KDAP antibody|HUMKER1A antibody|Insulin Stimulated MAP2 Kinase antibody|Insulin-stimulated MAP2 kinase antibody|MAP kinase 1 antibody|MAP kinase 3 antibody|MAP Kinase antibody|MAP kinase isoform p44 antibody|MAPK 1 antibody|MAPK 3 antibody|MAPK antibody|MAPK1 antibody|Mapk3 antibody|MGC20180 antibody|Microtubule Associated Protein 2 Kinase antibody|Microtubule-associated protein 2 kinase antibody|Mitogen Activated Protein Kinase 3 antibody|Mitogen-activated protein kinase 1 antibody|Mitogen-activated protein kinase 3 antibody|MK03_HUMAN antibody|OTTHUMP00000174538 antibody|OTTHUMP00000174541 antibody|p44 ERK1 antibody|p44 MAPK antibody|p44-ERK1 antibody|p44-MAPK antibody|P44ERK1 antibody|P44MAPK antibody|PRKM 3 antibody|PRKM3 antibody|Protein Kinase Mitogen Activated 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ERK1 antibody, ASA-B0665, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: COLO320 Cell Lysate
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: COLO320 Cell Lysate
Anti-ERK1 antibody, ASA-B0665, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
