More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hydroxysteroid (17-beta) dehydrogenase 10 | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSD17B10 |
Protein | 3-hydroxyacyl-CoA dehydrogenase type-2 |
Uniprot ID | Q99714 |
Function | Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). |
Tissue Specificity | Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. |
Sub-cellular localization | Mitochondrion. |
Sequence Similarities | |
Aliases | 17 beta hydroxysteroid dehydrogenase 10 antibody|17 beta hydroxysteroid dehydrogenase type 10 antibody|17-beta-HSD 10 antibody17-beta-hydroxysteroid dehydrogenase 10 antibody|17b HSD10 antibody|3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody|3 hydroxyacyl CoA dehydrogenase type 2 antibody|3 hydroxyacyl CoA dehydrogenase type II antibody|3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody|3-hydroxyacyl-CoA dehydrogenase type II antibody|3-hydroxyacyl-CoA dehydrogenase type-2 antibody|AB binding alcohol dehydrogenase antibody|ABAD antibody|Ads9 antibody|Amyloid beta binding polypeptide antibody|Amyloid beta peptide binding alcohol dehydrogenase antibody|Amyloid beta peptide binding protein antibody|CAMR antibody|DUPXp11.22 antibody|Endoplasmic Reticulum Amyloid Binding Protein antibody|Endoplasmic reticulum associated amyloid beta peptide binding protein antibody|Endoplasmic reticulum-associated amyloid beta-peptide-binding protein antibody|ER associated amyloid beta-binding protein antibody|ERAB antibody|HADH 2 antibody|HADH2 antibody|HCD 2 antibody|HCD2 antibody|HCD2_HUMAN antibody|Hsd17b10 antibody|Hydroxyacyl CoA Dehydrogenase type II antibody|Hydroxyacyl Coenzyme A dehydrogenase type II antibody|Hydroxysteroid (17 beta) dehydrogenase 10 antibody|Mental retardation X linked syndromic 11 antibody|MHBD antibody|Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase antibody|Mitochondrial ribonuclease P protein 2 antibody|Mitochondrial RNase P protein 2 antibody|MRPP2 antibody|MRX17 antibody|SCHAD antibody|SDR5C1 antibody|Short chain dehydrogenase/reductase family 5C member 1 antibody|Short chain L 3 hydroxyacyl CoA dehydrogenase type 2 antibody|Short chain type dehydrogenase/reductase XH98G2 antibody|Short-chain type dehydrogenase/reductase XH98G2 antibody|Type 10 17b HSD antibody|Type 10 17beta hydroxysteroid dehydrogenase antibody|Type II HADH antibody|XH98G2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ERAB antibody, ASA-B0662, Western blotting
All lanes: Anti ERAB (ASA-B0662) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: 293T Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
All lanes: Anti ERAB (ASA-B0662) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: 293T Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Anti- ERAB antibody, ASA-B0662,IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
