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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| EPH receptor A1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A1(963-976aa HQKRILCSIQGFKD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EPHA1 |
Protein | Ephrin type-A receptor 1(hEpha1) |
Uniprot ID | P21709 |
Function | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. |
Tissue Specificity | Overexpressed in several carcinomas. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. |
Aliases | EPH antibody|Eph tyrosine kinase 1 antibody|EphA1 antibody|Ephrin receptor Eph A1 antibody|Ephrin type A receptor 1 antibody|EPHT 1 antibody|EPHT antibody|Erythropoietin producing hepatoma amplified sequence antibody|Esk antibody|MGC163163 antibody|Oncogene EPH antibody|Soluble EPHA1 variant 1 antibody|Soluble EPHA1 variant 2 antibody|Tyrosine protein kinase receptor EPH antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Eph receptor A1 antibody, ASA-B0655, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: U87 Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: SW620 Cell Lysate
Lane 9: HELA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: U87 Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: COLO320 Cell Lysate
Lane 8: SW620 Cell Lysate
Lane 9: HELA Cell Lysate
Anti-Eph receptor A1 antibody, ASA-B0655, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Eph receptor A1 antibody, ASA-B0655, IHC(P)
IHC(P): Human Colon Cancer Tissue
IHC(P): Human Colon Cancer Tissue