EIF6  Antibody View larger

EIF6 Antibody

ASA-B0643

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

eukaryotic translation initiation factor 6 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

EIF6

Protein

Eukaryotic translation initiation factor 6

Uniprot ID

P56537

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.

Tissue Specificity

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

Sub-cellular localization

Cytoplasm. Nucleus, nucleolus. Note: Shuttles between cytoplasm and nucleus/nucleolus.

Sequence Similarities

Aliases

b(2)gcn antibody|B(2)GCN homolog antibody|B4 integrin interactor antibody|Binding protein of beta-4 integrin antibody|CAB antibody|eIF-6 antibody|EIF3A antibody|EIF6 antibody|Eukaryotic translation initiation factor 3A antibody|Eukaryotic translation initiation factor 6 antibody| IF6_HUMAN antibody|ITGB4BP antibody|OK/SW-cl.27 antibody|p27 beta 4 integrin binding protein antibody|p27(BBP) antibody|p27BBP antibody|RP4-614O4.1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EIF6 antibody, ASA-B0643, Western blotting
All lanes: Anti EIF6 (ASA-B0643) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Anti- EIF6 antibody, ASA-B0643,IHC(P)
IHC(P): Mouse Intestine Tissue
Anti- EIF6 antibody, ASA-B0643,IHC(P)
IHC(P): Rat Testis Tissue

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