More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EIF2S1 |
Protein | Eukaryotic translation initiation factor 2 subunit 1 |
Uniprot ID | P05198 |
Function | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasmic granule . Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). |
Sequence Similarities | Belongs to the eIF-2-alpha family. |
Aliases | EIF 2 alpha antibody|EIF 2 antibody|EIF 2A antibody|EIF 2alpha antibody|eIF-2-alpha antibody|eIF-2A antibody|EIF-2alpha antibody|EIF2 alpha antibody|EIF2 antibody|EIF2A antibody|EIF2S1 antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha antibody|Eukaryotic translation initiation factor 2 subunit 1 antibody|Eukaryotic translation initiation factor 2 subunit alpha antibody|IF2A_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EIF2S1 antibody, ASA-B0640, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 1: COLO320 Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: HT1080 Cell Lysate
Anti-EIF2S1 antibody, ASA-B0640, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-EIF2S1 antibody, ASA-B0640, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue