More info
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| early growth response 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Egr1(23-43aa FPHSPTMDNYPKLEEMMLLSN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EGR1 |
Protein |
Early growth response protein 1 |
Uniprot ID |
P18146 |
Function |
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the EGR C2H2-type zinc-finger protein family. |
Aliases |
AT 225 antibody|AT225 antibody|Early growth response 1 antibody|Early growth response protein 1 antibody|EGR 1 antibody|EGR-1 antibody|EGR1 antibody|EGR1_HUMAN antibody|G0S 30 antibody|G0S30 antibody|KROX 24 antibody|Krox 24 protein antibody|KROX24 antibody|Nerve growth factor induced protein A antibody|Nerve growth factor-induced clone A antibody|Nerve growth factor-induced protein A antibody|NGFI A antibody|NGFI-A antibody|NGFIA antibody|TIS 8 antibody|TIS8 antibody|Transcription factor ETR 103 antibody|Transcription factor ETR103 antibody|Transcription factor Zif 268 antibody|Transcription factor Zif268 antibody|Zfp 6 antibody|ZIF 268 antibody|ZIF268 antibody|Zinc finger protein 225 antibody|Zinc finger protein Krox-24 antibody|ZNF 225 antibody|ZNF225 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Egr1 antibody, ASA-B0637, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: COLO320 Cell Lysate
Anti-Egr1 antibody, ASA-B0637, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
Anti-Egr1 antibody, ASA-B0637, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue