EGF Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
epidermal growth factor	Polyclonal	IgG	Rabbit	Mouse	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR).

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	EGF
Protein	Pro-epidermal growth factor(EGF)
Uniprot ID	P01132
Function	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
Tissue Specificity
Sub-cellular localization	Membrane; Single-pass type I membrane protein.
Sequence Similarities	Contains 9 EGF-like domains.
Aliases	Beta urogastrone antibody|EGF antibody|Egf antibody|EGF_HUMAN antibody|Epidermal Growth Factor antibody|Epidermal Growth Factor antibody|HOMG4 antibody|OTTHUMP00000219721 antibody|OTTHUMP00000219722 antibody|Pro epidermal growth factor antibody|URG antibody|Urogastrone antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information