More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| epidermal growth factor | Polyclonal | IgG | Rabbit | Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EGF |
Protein | Pro-epidermal growth factor(EGF) |
Uniprot ID | P01132 |
Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 9 EGF-like domains. |
Aliases | Beta urogastrone antibody|EGF antibody|Egf antibody|EGF_HUMAN antibody|Epidermal Growth Factor antibody|Epidermal Growth Factor antibody|HOMG4 antibody|OTTHUMP00000219721 antibody|OTTHUMP00000219722 antibody|Pro epidermal growth factor antibody|URG antibody|Urogastrone antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EGF antibody, ASA-B0635, Western blotting
Lane 1: Recombinant Mouse EGF Protein 10ng
Lane 2: Recombinant Mouse EGF Protein 5ng
Lane 3: Recombinant Mouse EGF Protein 2.5ng
Lane 1: Recombinant Mouse EGF Protein 10ng
Lane 2: Recombinant Mouse EGF Protein 5ng
Lane 3: Recombinant Mouse EGF Protein 2.5ng
Anti-EGF antibody, ASA-B0635, IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
