EDNRB Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
endothelin receptor type B	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB (406-439aa QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY), different from the related mouse and rat sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	EDNRB
Protein	Endothelin B receptor
Uniprot ID	P24530
Function	Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue Specificity	Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
Sub-cellular localization	Cell membrane; Multi-pass membrane protein.
Sequence Similarities	Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
Aliases	ABCDS antibody|Ednra antibody|Ednrb antibody|EDNRB_HUMAN antibody| Endothelin B receptor antibody|Endothelin B receptor precursor antibody| Endothelin receptor Non selective type antibody|Endothelin receptor non-selective type antibody|Endothelin receptor type B antibody|ET B antibody|ET-B antibody|ET-BR antibody|ETB antibody|ETBR antibody|ETRB antibody|Hirschsprung disease 2 antibody|HSCR antibody|HSCR2 antibody|OTTHUMP00000018534 antibody| OTTHUMP00000178736 antibody|WS4A antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information