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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| endothelin receptor type B | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human EDNRB (406-439aa QSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKY), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EDNRB |
Protein | Endothelin B receptor |
Uniprot ID | P24530 |
Function | Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
Tissue Specificity | Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. |
Aliases | ABCDS antibody|Ednra antibody|Ednrb antibody|EDNRB_HUMAN antibody| Endothelin B receptor antibody|Endothelin B receptor precursor antibody| Endothelin receptor Non selective type antibody|Endothelin receptor non-selective type antibody|Endothelin receptor type B antibody|ET B antibody|ET-B antibody|ET-BR antibody|ETB antibody|ETBR antibody|ETRB antibody|Hirschsprung disease 2 antibody|HSCR antibody|HSCR2 antibody|OTTHUMP00000018534 antibody| OTTHUMP00000178736 antibody|WS4A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EDNRB antibody, ASA-B0633, Western blotting
All lanes: Anti EDNRB (ASA-B0633) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti EDNRB (ASA-B0633) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- EDNRB antibody, ASA-B0633, IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti- EDNRB antibody, ASA-B0633, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue