More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| estrogen receptor binding site associated, antigen, 9 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human EBAG9 recombinant protein (Position: R31-S213). Human EBAG9 shares 97.8% and 94.5% amino acid (aa) sequence identity with mouse and rat EBAG9, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EBAG9 |
Protein | Receptor-binding cancer antigen expressed on SiSo cells |
Uniprot ID | O00559 |
Function | May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases. |
Tissue Specificity | Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs. |
Sub-cellular localization | Golgi apparatus membrane. |
Sequence Similarities | |
Aliases | BAG9 antibody|Cancer associated surface antigen antibody|Cancer associated surface antigen RCAS1 antibody|Cancer-associated surface antigen RCAS1 antibody|EB9 antibody|EBAG 9 antibody|EBAG9 antibody|Estrogen receptor binding fragment associated gene 9 protein antibody|Estrogen receptor binding site associated antigen 9 antibody|estrogen receptor binding site associated, antigen, 9 antibody|Estrogen receptor-binding fragment-associated gene 9 protein antibody|PDAF antibody|RCAS 1 antibody|RCAS1 antibody| RCAS1_ HUMAN antibody| Receptor binding cancer antigen expressed on SiSo cells antibody|Receptor-binding cancer antigen expressed on SiSo cells antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EBAG9 antibody, ASA-B0630, Western blotting
All lanes: Anti EBAG9 (ASA-B0630) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
All lanes: Anti EBAG9 (ASA-B0630) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Anti- EBAG9 antibody, ASA-B0630,IHC(P)
IHC(P): Mouse Testis Tissue
IHC(P): Mouse Testis Tissue
Anti- EBAG9 antibody, ASA-B0630,IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue