More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 1(glial high affinity glutamate transporter), member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1(519-537aa MKKPYQLIAQDNETEKPID), different from the related rat and mouse sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC1A3 |
Protein | Excitatory amino acid transporter 1 |
Uniprot ID | P43003 |
Function | Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. |
Tissue Specificity | Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | EA6 antibody|EAA1_HUMAN antibody|EAAT1 antibody|Excitatory amino acid transporter 1 antibody|FLJ25094 antibody|GLAST antibody|GLAST-1 antibody|GLAST1 antibody|Glial high affinity glutamate transporter antibody|glutamate/aspartate transporter, high affinity, sodium-dependent antibody|High affinity neuronal glutamate transporter antibody|Slc1a3 antibody|Sodium dependent glutamate/aspartate transporter antibody|Sodium-dependent glutamate/aspartate transporter 1 antibody|Solute carrier family 1(glial high affinity glutamate transporter) member 3 antibody|Solute carrier family 1 member 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EAAT1 antibody, ASA-B0625, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Anti-EAAT1 antibody, ASA-B0625, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
