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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor receptor superfamily, member 10b | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DR5(333-348aa DPTETLRQCFDDFADL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFRSF10B |
Protein | Tumor necrosis factor receptor superfamily member 10B |
Uniprot ID | O14763 |
Function | Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Essential for ER stress-induced apoptosis. |
Tissue Specificity | Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 1 death domain. |
Aliases | Fas like protein antibody|Apoptosis inducing protein TRICK2A/2B antibody|Apoptosis inducing receptor TRAIL R2 antibody|CD262 antibody|CD262 antigen antibody|Cytotoxic TRAIL receptor 2 antibody|Death domain containing receptor for TRAIL/Apo 2L antibody|Death receptor 5 antibody|DR5 antibody|KILLER antibody|KILLER/DR5 antibody|OTTHUMP00000123492 antibody|OTTHUMP00000123493 antibody|p53 regulated DNA damage inducible cell death receptor(killer) antibody|TNF related apoptosis inducing ligand receptor 2 antibody|TNF-related apoptosis-inducing ligand receptor 2 antibody|TNFRSF10B antibody|TR10B_HUMAN antibody|TRAIL R2 antibody|TRAIL receptor 2 antibody|TRAIL-R2 antibody|TRAILR2 antibody|TRICK2 antibody|TRICK2A antibody|TRICK2B antibody|TRICKB antibody|Tumor necrosis factor receptor like protein ZTNFR9 antibody|Tumor necrosis factor receptor superfamily member 10B antibody|Tumor necrosis factor receptor superfamily, member 10b antibody|ZTNFR9 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DR5 antibody, ASA-B0610, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: SGC Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: SGC Cell Lysate
Lane 4: HT1080 Cell Lysate
Anti-DR5 antibody, ASA-B0610, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
