More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| doublesex and mab-3 related transcription factor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1 (98-128aa RDCQCKKCNLIAERQRVMAAQVALRRQQAQE), different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DMRT1 |
Protein | Doublesex- and mab-3-related transcription factor 1 |
Uniprot ID | Q9Y5R6 |
Function | Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis (By similarity). |
Tissue Specificity | Testis-specific. Expressed in prostate cancer (at protein level). |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the DMRT family. |
Aliases | CT154 antibody|DM domain expressed in testis 1 antibody|DM domain expressed in testis protein 1 antibody|DM domain gene expressed in testis antibody|DMRT 1 antibody|dmrt1 antibody|DMRT1_HUMAN antibody|DMT 1 antibody|DMT1 antibody|Doublesex and mab 3 related transcription factor 1 antibody|Doublesex- and mab-3-related transcription factor 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DMRT1 antibody, ASA-B0600, Western blotting
All lanes: Anti DMRT1 (ASA-B0600) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti DMRT1 (ASA-B0600) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- DMRT1 antibody, ASA-B0600,IHC(P)
IHC(P): Human Testis Tissue
IHC(P): Human Testis Tissue
