DLD  Antibody View larger

DLD Antibody

ASA-B0597

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

dihydrolipoamide dehydrogenase Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

DLD

Protein

Dihydrolipoyl dehydrogenase, mitochondrial

Uniprot ID

P09622

Function

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Tissue Specificity

Sub-cellular localization

Mitochondrion matrix.

Sequence Similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Aliases

Diaphorase antibody|Dihydrolipoamide dehydrogenase antibody|Dihydrolipoyl dehydrogenase antibody|Dihydrolipoyl dehydrogenase mitochondrial antibody|dld antibody|DLDH antibody|DLDH_HUMAN antibody|E3 antibody|E3 component of pyruvate dehydrogenase antibody| E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody|GCSL antibody|Glycine cleavage system L protein antibody|Glycine cleavage system protein L antibody|LAD antibody|lipoamide dehydrogenase antibody|Lipoamide reductase antibody|Lipoyl dehydrogenase antibody|mitochondrial antibody|OTTHUMP00000206744 antibody| OTTHUMP00000206746 antibody|OTTHUMP00000206748 antibody|OTTHUMP00000206749 antibody|PHE 3 antibody|PHE3 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DLD antibody, ASA-B0597, Western blotting
All lanes: Anti DLD (ASA-B0597) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
Anti- DLD antibody, ASA-B0597, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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