More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dickkopf WNT signaling pathway inhibitor 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human Dkk3 recombinant protein (Position: A22-D152). Human Dkk3 shares 85.5% amino acid (aa) sequence identity with mouse Dkk3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DKK3 |
Protein | Dickkopf-related protein 3 |
Uniprot ID | Q9UBP4 |
Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). |
Tissue Specificity | Highest expression in heart, brain, and spinal cord. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Dickkopf 3 antibody|Dickkopf 3 homolog (Xenopus laevis) antibody|Dickkopf 3 homolog antibody|Dickkopf homolog 3 antibody|Dickkopf related protein 3 antibody|Dickkopf related protein 3 Precursor antibody|Dickkopf WNT signaling pathway inhibitor 3 antibody|Dickkopf, Xenopus, homolog of, 3 antibody|Dickkopf-3 antibody|Dickkopf-related protein 3 antibody| Dickkopf3 antibody|DKK 3 antibody|Dkk-3 antibody|DKK3 antibody|DKK3_HUMAN antibody|hDkk 3 antibody| hDkk-3 antibody|hDkk3 antibody|Regulated in glioma antibody|REIC antibody|RIG antibody|RIG-like 5-6 antibody|RIG-like 7-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DKK3 antibody, ASA-B0596, Western blotting
All lanes: Anti DKK3 (ASA-B0596) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti DKK3 (ASA-B0596) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- DKK3 antibody, ASA-B0596, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- DKK3 antibody, ASA-B0596, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue