More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dicer 1, ribonuclease type III | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DICER1 |
Protein | Endoribonuclease Dicer |
Uniprot ID | Q9UPY3 |
Function | Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA- induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the helicase family. Dicer subfamily. |
Aliases | DCR antibody|DCR1 antibody|Dcr1 homolog (Drosophila) antibody|Dicer 1 antibody|Dicer 1 ribonuclease type III antibody|Dicer antibody|DICER_HUMAN antibody|Dicer1 antibody|Double-strand-specific ribonuclease antibody|Endoribonuclease Dicer antibody|Helicase moi antibody|Helicase with RNAse motif antibody|HERNA antibody|KIAA0928 antibody|MNG1 antibody|RMSE2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Dicer antibody, ASA-B0590, Western blotting
All lanes: Anti Dicer (ASA-B0590) at 0.5ug/ml
WB: Recombinant Human Dicer Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Dicer (ASA-B0590) at 0.5ug/ml
WB: Recombinant Human Dicer Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- Dicer antibody, ASA-B0590, Western blotting
All lanes: Anti Dicer (ASA-B0590) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 217KD
Observed bind size: 217KD
All lanes: Anti Dicer (ASA-B0590) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 217KD
Observed bind size: 217KD
Anti- Dicer antibody, ASA-B0590, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue