More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| dihydrofolate reductase | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DHFR |
Protein | Dihydrofolate reductase |
Uniprot ID | P00374 |
Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. |
Tissue Specificity | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
Sub-cellular localization | |
Sequence Similarities | Belongs to the dihydrofolate reductase family. |
Aliases | DHFR antibody|DHFRP1 antibody|Dihydrofolate reductase antibody|DYR antibody|DYR_HUMAN antibody|EC 1.5.1.3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DHFR antibody, ASA-B0589, Western blotting
All lanes: Anti DHFR (ASA-B0589) at 0.5ug/ml
WB: Recombinant Human DHFR Protein 0.5ng
Predicted bind size: 32KD
Observed bind size: 32KD
All lanes: Anti DHFR (ASA-B0589) at 0.5ug/ml
WB: Recombinant Human DHFR Protein 0.5ng
Predicted bind size: 32KD
Observed bind size: 32KD
Anti- DHFR antibody, ASA-B0589, Western blotting
All lanes: Anti DHFR (ASA-B0589) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HepG2 Whole Cell Lysate at 40ug
Lane 4: Jurkat Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
All lanes: Anti DHFR (ASA-B0589) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HepG2 Whole Cell Lysate at 40ug
Lane 4: Jurkat Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Anti- DHFR antibody, ASA-B0589,IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue