More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| desmoglein 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 2(1104-1118aa STRVTKHSTVQHSYS), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DSG2 |
Protein | Desmoglein-2 |
Uniprot ID | Q14126 |
Function | Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. |
Tissue Specificity | All of the tissues tested and carcinomas. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome. |
Sequence Similarities | Contains 4 cadherin domains. |
Aliases | ARVC 10 antibody|ARVC10 antibody|ARVD 10 antibody|ARVD10 antibody|Cadherin family member 5 antibody|CDHF 5 antibody|CDHF5 antibody|CMD1BB antibody|Desmoglein 2 antibody|Desmoglein-2 antibody|Desmoglein2 antibody|DSG 2 antibody|DSG2 antibody|DSG2_HUMAN antibody|HDGC antibody|HDGC included antibody|MGC117034 antibody|MGC117036 antibody|MGC117037 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Desmoglein 2 antibody, ASA-B0587, Western blotting
All lanes: Anti Desmoglein 2 (ASA-B0587) at 0.5ug/ml
Lane 1: HT1080 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: SCG Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
All lanes: Anti Desmoglein 2 (ASA-B0587) at 0.5ug/ml
Lane 1: HT1080 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: SCG Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
