Desmin Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
desmin	Monoclonal	IgG	Mouse	Human, Mouse, Rabbit, Rat	IHC-P, WB	Ascites
Immunogen
Desmin from pig stomach.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	DES
Protein	Desmin
Uniprot ID	Human(P17661), Mouse(P31001), Rat(P48675)
Function	Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
Tissue Specificity
Sub-cellular localization	Cytoplasm. Cell membrane, sarcolemma.
Sequence Similarities	Belongs to the intermediate filament family.
Aliases	CMD1I|CSM1|CSM2|intermediate filament protein

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	2μg/ml	Human, mouse, rat, rabbit	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	2-4μg/ml	Human, mouse, rat, rabbit	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information