More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| decorin | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Decorin recombinant protein (Position: D31-K359). Human Decorin shares 80% and 77% amino acid (aa) sequences identity with mouse and rat Decorin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DCN |
Protein | Decorin |
Uniprot ID | P07585 |
Function | May affect the rate of fibrils formation. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. |
Aliases | Bone proteoglycan II antibody|CSCD antibody|DCN antibody|DCN protein antibody|Decorin antibody|Decorin proteoglycan antibody|Dermatan sulphate proteoglycans II antibody|DKFZp686J19238 antibody|DSPG 2 antibody|DSPG2 antibody|PG 40 antibody|PG II antibody|PG S2 antibody|PG-S2 antibody|PG40 antibody|PGII antibody|PGS 2 antibody|PGS2 antibody|PGS2_HUMAN antibody|Proteoglycan core protein antibody|SLRR1B antibody|Small leucine rich protein 1B antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Decorin antibody, ASA-B0584, Western blotting
All lanes: Anti Decorin (ASA-B0584) at 0.5ug/ml
WB: Recombinant Human Decorin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti Decorin (ASA-B0584) at 0.5ug/ml
WB: Recombinant Human Decorin Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- Decorin antibody, ASA-B0584, Western blotting
All lanes: Anti Decorin (ASA-B0584) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Decorin (ASA-B0584) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- Decorin antibody, ASA-B0584, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue