DDX5 Antibody View larger

DDX5 Antibody

ASA-B0581

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

DEAD(Asp-Glu-Ala-Asp) box helicase 5 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5(566-580aa TYQNGYDSTQQYGSN), identical to the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

DDX5

Protein

Probable ATP-dependent RNA helicase DDX5

Uniprot ID

P17844

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

Tissue Specificity

Sub-cellular localization

Nucleus, nucleolus.

Sequence Similarities

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.

Aliases

ATP dependent RNA helicase DDX5 antibody|DDX 5 antibody|Ddx5 antibody|DDX5_HUMAN antibody|DEAD(Asp Glu Ala Asp) box helicase 5 antibody|DEAD(Asp Glu Ala Asp) box polypeptide 5 antibody|DEAD box 5 antibody|DEAD box protein 5 antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5(RNA helicase, 68kD) antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 5(RNA helicase 68kDa) antibody|DEAD/H box-5(RNA helicase 68kD) antibody|DKFZp686J01190 antibody|G17P1 antibody|HELR antibody|HLR 1 antibody|HLR1 antibody|HUMP68 antibody|P68 antibody|p68 RNA helicase antibody|Probable ATP dependent RNA helicase DDX5 antibody|Probable ATP-dependent RNA helicase DDX5 antibody|RNA helicase 68kD antibody|RNA helicase p68 antibody|RNA-dependent ATPase antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDX5 antibody, ASA-B0581, Western blotting
All lanes: Anti DDX5 (ASA-B0581) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
Anti-DDX5 antibody, ASA-B0581, IHC(P)
IHC(P): Rat Brain Tissue

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