DDX4/MVH Antibody View larger

DDX4/MVH Antibody

ASA-B0580

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

DEAD(Asp-Glu-Ala-Asp) box polypeptide 4 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

DDX4

Protein

Probable ATP-dependent RNA helicase DDX4

Uniprot ID

Q9NQI0

Function

May play a role in germ cell development. May play a role in sperm motility.

Tissue Specificity

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the DEAD box helicase family. DDX4/VASA subfamily.

Aliases

DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human Mouse, RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDX4/MVH antibody, ASA-B0580, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: HELA Cell Lysate
Anti-DDX4/MVH antibody, ASA-B0580, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Mouse Testis Tissue Lysate
Lane 3: Mouse Ovary Tissue Lysate
Anti-DDX4/MVH antibody, ASA-B0580, IHC(P)
IHC(P): Rat Ovary Tissue

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