DDX4/MVH Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
DEAD(Asp-Glu-Ala-Asp) box polypeptide 4	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	DDX4
Protein	Probable ATP-dependent RNA helicase DDX4
Uniprot ID	Q9NQI0
Function	May play a role in germ cell development. May play a role in sperm motility.
Tissue Specificity	Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Aliases	DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information