More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| D-dopachrome tautomerase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DDT(99-118aa RFFPLESWQIGKIGTVMTFL), different from the related mouse and rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DDT |
Protein | D-dopachrome decarboxylase |
Uniprot ID | P30046 |
Function | Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm . |
Sequence Similarities | |
Aliases | D dopachrome decarboxylase antibody|D dopachrome tautomerase antibody|D-dopachrome decarboxylase antibody|D-dopachrome tautomerase antibody|DDCT antibody|Ddt antibody|Phenylpyruvate tautomerase II antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDT antibody, ASA-B0579, Western blotting
Lane 1: Recombinant Human DDT Protein 10ng
Lane 2: Recombinant Human DDT Protein 5ng
Lane 3: Recombinant Human DDT Protein 2.5ng
Lane 4: Recombinant Human DDT Protein 1.25ng
Lane 1: Recombinant Human DDT Protein 10ng
Lane 2: Recombinant Human DDT Protein 5ng
Lane 3: Recombinant Human DDT Protein 2.5ng
Lane 4: Recombinant Human DDT Protein 1.25ng
Anti-DDT antibody, ASA-B0579, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
