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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| damage-specific DNA binding protein 1, 127kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DDB1 |
Protein | DNA damage-binding protein 1 |
Uniprot ID | Q16531 |
Function | Required for DNA repair. Binds to DDB2 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. |
Sequence Similarities | Belongs to the DDB1 family. |
Aliases | Damage specific DNA binding protein 1 antibody|Damage-specific DNA-binding protein 1 antibody|DDB 1 antibody|DDB p127 subunit antibody| Ddb1 antibody|DDB1_HUMAN antibody|DDBa antibody|DNA damage binding protein 1 antibody|DNA damage-binding protein 1 antibody|DNA damage-binding protein a antibody|HBV X-associated protein 1 antibody|UV damaged DNA binding factor antibody|UV damaged DNA binding protein 1 antibody|UV DDB 1 antibody|UV DDB1 antibody|UV-damaged DNA-binding factor antibody|UV-damaged DNA-binding protein 1 antibody|UV-DDB 1 antibody|UV-DDB1 antibody|X associated protein 1 antibody|XAP 1 antibody|XAP-1 antibody|XAP1 antibody|Xeroderma pigmentosum group E complementing protein antibody|Xeroderma pigmentosum group E-complementing protein antibody|XPCE antibody| XPE antibody|XPE BF antibody|XPE binding factor antibody|XPE-BF antibody|XPE-binding factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDB1 antibody, ASA-B0577, Western blotting
All lanes: Anti DDB1 (ASA-B0577) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Ovary Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
All lanes: Anti DDB1 (ASA-B0577) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Ovary Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 127KD
Observed bind size: 127KD
Anti-DDB1 antibody, ASA-B0577, IHC(P)
IHC(P): Mouse Liver Tissue
IHC(P): Mouse Liver Tissue
Anti-DDB1 antibody, ASA-B0577, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue