More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| enoyl-CoA delta isomerase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DCI(272-290aa ADVQNFVSFISKDSIQKSL), different from the related mouse sequence by two amino acids and from the related rat sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ECI1 |
Protein | Enoyl-CoA delta isomerase 1, mitochondrial |
Uniprot ID | P42126 |
Function | Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion matrix. |
Sequence Similarities | |
Aliases | 3,2 trans enoyl Coenzyme A isomerase antibody|Acetylene allene isomerase antibody|D3,D2 enoyl CoA isomerase antibody|DCI antibody|DCI protein antibody|Delta(3),Delta(2) enoyl CoA isomerase antibody|Delta3, delta2 enoyl CoA isomerase antibody|Dodecenoyl CoA delta Isomerase antibody|Dodecenoyl CoA isomerase antibody|Dodecenoyl Coenzyme A delta isomerase antibody|Dodecenoyl Coenzyme A delta isomerase(3,2 trans enoyl Coenzyme A isomerase) antibody|Dodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase, isoform CRA a antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DCI antibody, ASA-B0576, Western blotting
All lanes: Anti DCI (ASA-B0576) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti DCI (ASA-B0576) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Anti-DCI antibody, ASA-B0576, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-DCI antibody, ASA-B0576, ICC
ICC: HELA Cell
ICC: HELA Cell