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Daxx Antibody

ASA-B0574

$365.00

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Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
death-domain associated protein Polyclonal IgG Rabbit Human, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

E.coli-derived human Daxx recombinant protein (Position: K56-R345). Human Daxx shares 88.3% and 88.6% amino acid (aa) sequence identity with mouse and rat Daxx, respectively.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 DAXX

Protein

 Death domain-associated protein 6

Uniprot ID

 Q9UER7

Function

 Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activatiopn of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upopn neuronal activation asociates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML- NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).

Tissue Specificity

Ubiquitous.

Sub-cellular localization

 Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body.

Sequence Similarities

 Belongs to the DAXX family.

Aliases

 BING 2 antibody|BING2 antibody|CENP-C binding protein antibody|DAP 6 antibody|DAP6 antibody|Daxx antibody|DAXX antibody|DAXX_HUMAN antibody| Death associated protein 6 antibody|Death domain associated protein 6 antibody| Death domain associated protein antibody|Death domain-associated protein 6 antibody|EAP 1 antibody|EAP1 antibody|ETS1 associated protein 1 antibody| ETS1-associated protein 1 antibody|Fas binding protein antibody|Fas death domain associated protein antibody|Fas death domain-associated protein antibody|hDaxx antibody|MGC126245 antibody|MGC126246 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, RatAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml HumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- DAXX antibody, ASA-B0574, Western blotting
All lanes: Anti DAXX (ASA-B0574) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 130KD
Anti- DAXX antibody, ASA-B0574,IHC(P)
IHC(P): Rat Intestine Tissue
Anti- DAXX antibody, ASA-B0574,IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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