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Cytokeratin Peptide 7 Antibody (monoclonal)

ASA-B0571

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

keratin 7 Monoclonal IgG Mouse Human IHC-P, IHC-F, WB Ascites

Immunogen

Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

KRT7

Protein

Keratin, type II cytoskeletal 7

Uniprot ID

Human(P08729), Mouse(Q9DCV7), Rat(Q6IG12)

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Tissue Specificity

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Sub-cellular localization

Cytoplasm.

Sequence Similarities

Belongs to the intermediate filament family.

Aliases

SCL|cytokeratin 7|K2C7|K7|keratin|Keratin-7|55K type II cytoskeletal|keratin, type II cytoskeletal 7|sarcolectin|KERATIN, SIMPLE EPITHELIAL|KERATIN, TYPE II, CYTOSKELETAL, 7|SARCOLECTIN|Type-II keratin Kb7

Application Details

Application Concentration* Species Validated Using**
Western blot1-2μg/mlHumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)2-4μg/mlHumanAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)2-4μg/mlHumanAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

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