More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| keratin 18 | Monoclonal | IgG | Mouse | Human, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen | ||||||
| The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KRT18 |
Protein | Keratin, type I cytoskeletal 18 |
Uniprot ID | Human(P05783), Mouse(P05784), Rat(Q5BJY9) |
Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
Tissue Specificity | Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
Sub-cellular localization | Cytoplasm, perinuclear region. Nucleus, nucleolus. |
Sequence Similarities | Belongs to the intermediate filament family. |
Aliases | K18|Keratin-18|CK-18|Cytokeratin-18|CYK18|Cell proliferation-inducing gene 46 protein |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-2μg/ml | Human, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
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Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, Western blotting
WB: MCF-7 Cell Lysate
WB: MCF-7 Cell Lysate
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Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
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Anti-Cytokeratin Peptide 18 antibody (monoclonal), ASA-B0570, IHC(P)
IHC(P): Human Rectal Cancer Tissue
IHC(P): Human Rectal Cancer Tissue