Cytokeratin 8 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
keratin 8	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8(127-145aa QQQKTARSNMDNMFESYIN), different from the related rat and mouse sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	KRT8
Protein	Keratin, type II cytoskeletal 8
Uniprot ID	P05787
Function	Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue Specificity	Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Sub-cellular localization	Cytoplasm.
Sequence Similarities	Belongs to the intermediate filament family.
Aliases	card2 antibody|Cardiac autoantigen 2 120kD antibody|CK 8 antibody|CK-8 antibody|ck8 antibody|Cyk 8 antibody|cyk8 antibody|CYKER antibody|Cytokeratin 8 antibody|Cytokeratin endo A antibody|Cytokeratin-8 antibody|Cytokeratin8 antibody|DreK8 antibody|EndoA antibody|k0 antibody|k2c8 antibody|K2C8_HUMAN antibody|k8 antibody|Keratin 8 antibody|Keratin type II cytoskeletal 8 antibody|Keratin, type II cytoskeletal 8 antibody|Keratin-8 antibody|Keratin8 antibody|KO antibody|Krt 2.8 antibody|Krt 8 antibody|krt8 antibody|MGC118110 antibody|MGC174782 antibody|MGC53564 antibody|MGC85764 antibody|sb:cb186 antibody|Type-II keratin Kb8 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information