More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| keratin 8 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Cytokeratin 8(127-145aa QQQKTARSNMDNMFESYIN), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KRT8 |
Protein | Keratin, type II cytoskeletal 8 |
Uniprot ID | P05787 |
Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
Tissue Specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the intermediate filament family. |
Aliases | card2 antibody|Cardiac autoantigen 2 120kD antibody|CK 8 antibody|CK-8 antibody|ck8 antibody|Cyk 8 antibody|cyk8 antibody|CYKER antibody|Cytokeratin 8 antibody|Cytokeratin endo A antibody|Cytokeratin-8 antibody|Cytokeratin8 antibody|DreK8 antibody|EndoA antibody|k0 antibody|k2c8 antibody|K2C8_HUMAN antibody|k8 antibody|Keratin 8 antibody|Keratin type II cytoskeletal 8 antibody|Keratin, type II cytoskeletal 8 antibody|Keratin-8 antibody|Keratin8 antibody|KO antibody|Krt 2.8 antibody|Krt 8 antibody|krt8 antibody|MGC118110 antibody|MGC174782 antibody|MGC53564 antibody|MGC85764 antibody|sb:cb186 antibody|Type-II keratin Kb8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytokeratin 8 antibody, ASA-B0569, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: COLO320 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: COLO320 Cell Lysate
Anti-Cytokeratin 8 antibody, ASA-B0569, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
