More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| keratin 19 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19(379-392aa YRSLLEGQEDHYNN). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KRT19 |
Protein | Keratin, type I cytoskeletal 19 |
Uniprot ID | P08727 |
Function | Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. |
Tissue Specificity | Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | 40 kDa keratin intermediate filament antibody|CK 19 antibody|CK-19 antibody|ck19 antibody|Cytokeratin 19 antibody|Cytokeratin-19 antibody|k19 antibody|K1C19_HUMAN antibody|k1cs antibody|Keratin 19 antibody|Keratin type I 40 kD antibody|Keratin type I 40kD antibody|Keratin type I cytoskeletal 19 antibody|Keratin, type I cytoskeletal 19 antibody|Keratin, type I, 40 kd antibody|Keratin-19 antibody|krt19 antibody|mgc15366 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytokeratin 19 antibody, ASA-B0568, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-Cytokeratin 19 antibody, ASA-B0568, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue
Anti-Cytokeratin 19 antibody, ASA-B0568, ICC
ICC: MCF-7 Cell
ICC: MCF-7 Cell