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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| P450(cytochrome) oxidoreductase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome P450 Reductase(659-677aa DYIKKLMTKGRYSLDVWS), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | POR |
Protein | NADPH--cytochrome P450 reductase |
Uniprot ID | P16435 |
Function | This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. |
Tissue Specificity | |
Sub-cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N- terminal hydrophobic region. |
Sequence Similarities | In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. |
Aliases | CPR antibody|CYPOR antibody|DKFZp686G04235 antibody|FLJ26468 antibody|NADPH Cytochrome P450 Reductase antibody|NADPH dependent cytochrome P450 reductase antibody|NADPH--cytochrome P450 reductase antibody|NCPR_HUMAN antibody|P450(cytochrome) oxidoreductase antibody|P450 Cytochrome Oxidoreductase antibody|P450R antibody|POR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytochrome P450 Reductase antibody, ASA-B0565, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Spleen Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SKOV Cell Lysate
Lane 8: MCF-7 Cell Lysate
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Spleen Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SKOV Cell Lysate
Lane 8: MCF-7 Cell Lysate
Anti-Cytochrome P450 Reductase antibody, ASA-B0565, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
Anti-Cytochrome P450 Reductase antibody, ASA-B0565, ICC
ICC: HELA Cell
ICC: HELA Cell