More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 1, subfamily A, polypeptide 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Cytochrome P450 1A2(265-282aa QKTVQEHYQDFDKNSVRD), different from the related rat and mouse sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP1A2 |
Protein | Cytochrome P450 1A2 |
Uniprot ID | P05177 |
Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin. |
Tissue Specificity | Liver. |
Sub-cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Sequence Similarities | Belongs to the cytochrome P450 family. |
Aliases | Aryl hydrocarbon hydroxylase antibody|CP 12 antibody|CP12 antibody|CP1A2_HUMAN antibody|CYP1A2 antibody|CYPIA2 antibody|Cytochrome P(3)450 antibody|Cytochrome P450 1A2 antibody|Cytochrome P450 4 antibody|Cytochrome P450 family 1 polypeptide 2 antibody|Cytochrome P450 family 1 subfamily A polypeptide 2 antibody|Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody|Cytochrome P450-P3 antibody|Cytochrome P4501A2 antibody|Dioxin inducable P3 450 antibody|Flavoprotein linked monooxygenase antibody|Microsomal monooxygenase antibody|P(3)450 antibody|P3 450 antibody|P450 4 antibody|P450 form 4 antibody|P450 P3 antibody|P450(PA) antibody|Xenobiotic monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cytochrome P450 1A2 antibody, ASA-B0561, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HEPA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HEPA Cell Lysate
Anti-Cytochrome P450 1A2 antibody, ASA-B0561, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
