More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome c, somatic | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYCS |
Protein | Cytochrome c |
Uniprot ID | P99999 |
Function | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion intermembrane space. Note: Loosely associated with the inner membrane. |
Sequence Similarities | Belongs to the cytochrome c family. |
Aliases | CYC antibody|CYC_HUMAN antibody|CYCS antibody|Cytochrome c antibody|Cytochrome c somatic antibody|HCS antibody|THC4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cytochrome C antibody, ASA-B0560, Western blotting
All lanes: Anti Cytochrome C (ASA-B0560) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: NEURO Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
L
All lanes: Anti Cytochrome C (ASA-B0560) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: NEURO Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
L
Anti- Cytochrome C antibody, ASA-B0560, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
Anti- Cytochrome C antibody, ASA-B0560, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue