More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystatin A (stefin A) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Cystatin A recombinant protein (Position: E8-F98). Human Cystatin A shares 61.5% and 58.2% amino acid (aa) sequence identity with mouse and rat Cystatin A, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CSTA |
Protein | Cystatin-A |
Uniprot ID | P01040 |
Function | This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. |
Tissue Specificity | Expressed in the skin throughout the epidermis. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the cystatin family. |
Aliases | AREI antibody|CSTA antibody|CSTA protein antibody|Cystatin A antibody|Cystatin AS antibody| Cystatin-A antibody|Cystatin-AS antibody|CystatinA antibody| CYTA_ HUMAN antibody| Stefin A antibody|Stefin A1 antibody|Stefin-A antibody|StefinA antibody|STF 1 antibody|STF1 antibody|STFA antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Cystatin A antibody, ASA-B0557, Western blotting
All lanes: Anti Cystatin A (ASA-B0557) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
All lanes: Anti Cystatin A (ASA-B0557) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 54KD
Observed bind size: 54KD
Anti- Cystatin A antibody, ASA-B0557, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
