More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystathionase(cystathionine gamma-lyase) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Cystathionase(112-131aa DVYGGTNRYFRQVASEFGLK), different from the related rat and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTH |
Protein | Cystathionine gamma-lyase |
Uniprot ID | P32929 |
Function | Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | CGL_HUMAN antibody|CTH antibody|cystathionase(cystathionine gamma-lyase) antibody|Cystathionine gamma lyase antibody|Cystathionine gamma-lyase antibody|Cysteine desulfhydrase antibody|Gamma cystathionase antibody|Gamma-cystathionase antibody|Homoserine deaminase antibody|Homoserine dehydratase antibody|MGC9471 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cystathionase antibody, ASA-B0554, IHC(P)
IHC(P): Rat Liver Tissue
IHC(P): Rat Liver Tissue
Anti-Cystathionase antibody, ASA-B0554, ICC
ICC: SMMC Cell
ICC: SMMC Cell
Anti-Cystathionase antibody, ASA-B0554,Western blotting
All lanes: Anti Cystathionase (ASA-B0554) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 44 KD
Observed bind size: 44 KD
All lanes: Anti Cystathionase (ASA-B0554) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Predicted bind size: 44 KD
Observed bind size: 44 KD