More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 2, subfamily U, polypeptide 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CYP2U1(457-473aa EKPEDFYPNRFLDDQGQ). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP2U1 |
Protein | Cytochrome P450 2U1 |
Uniprot ID | Q7Z449 |
Function | Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes. |
Tissue Specificity | Widely expressed with stronger expression in thymus, heart and cerebellum. |
Sub-cellular localization | Endoplasmic reticulum membrane. |
Sequence Similarities | |
Aliases | CP2U1_HUMAN antibody|CYP2U 1 antibody|CYP2U1 antibody|Cytochrome P450 2U1 antibody|Cytochrome P450, family 2, subfamily U, polypeptide 1 antibody|EC 1.14.14.1 antibody|P450TEC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CYP2U1 antibody, ASA-B0550, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: HT1080 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: HT1080 Cell Lysate
Anti-CYP2U1 antibody, ASA-B0550, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
