More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 1, subfamily B, polypeptide 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CYP1B1 recombinant protein (Position: R255-L480). Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP1B1 |
Protein | Cytochrome P450 1B1 |
Uniprot ID | Q16678 |
Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta- estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression. |
Tissue Specificity | Expressed in many tissues. |
Sub-cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion . |
Sequence Similarities | Belongs to the cytochrome P450 family. |
Aliases | Aryl hydrocarbon hydroxylase antibody|CP1B antibody|CP1B1_HUMAN antibody| Cyp1b1 antibody| CYPIB1 antibody|Cytochrome P450 1B1 antibody|Cytochrome P450 family 1 subfamily B polypeptide 1 antibody|Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody| Flavoprotein linked monooxygenase antibody|GLC3A antibody|Microsomal monooxygenase antibody|P4501B1 antibody|Xenobiotic monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CYP1B1 antibody, ASA-B0546, Western blotting
All lanes: Anti CYP1B1 (ASA-B0546) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
All lanes: Anti CYP1B1 (ASA-B0546) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
Anti- CYP1B1 antibody, ASA-B0546,IHC(P)
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue
Anti- CYP1B1 antibody, ASA-B0546,IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue