More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cytochrome P450, family 1, subfamily A, polypeptide 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CYP1A1 |
Protein | Cytochrome P450 1A1 |
Uniprot ID | P04798 |
Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
Tissue Specificity | Lung, lymphocytes and placenta. |
Sub-cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Sequence Similarities | Belongs to the cytochrome P450 family. |
Aliases | AHH antibody|AHRR antibody|Aryl hydrocarbon hydroxylase antibody|CP 11 antibody|CP11 antibody|CP1A1_HUMAN antibody|CYP 1 antibody|CYP1 antibody|CYP1A1 antibody|CYPIA1 antibody| Cytochrome P1 450 dioxin inducible antibody|Cytochrome P1-450 antibody|Cytochrome P1-450 dioxin-inducible antibody|Cytochrome P450 1A1 antibody|Cytochrome P450 family 1 subfamily A polypeptide 1 antibody|Cytochrome P450 form 6 antibody| Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody|Cytochrome P450 subfamily I aromatic compound inducible polypeptide 1 antibody|Cytochrome P450-C antibody|Cytochrome P450-P1 antibody|Flavoprotein-linked monooxygenase antibody|Microsomal monooxygenase antibody|P1 450 antibody|P450 C antibody|P450 form 6 antibody|P450 P1 antibody|P450DX antibody| Xenobiotic monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CYP1A1 antibody, ASA-B0544, Western blotting
All lanes: Anti CYP1A1 (ASA-B0544) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
All lanes: Anti CYP1A1 (ASA-B0544) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
Anti- CYP1A1 antibody, ASA-B0544,IHC(P)
IHC(P): Mouse Kidney Tissue
IHC(P): Mouse Kidney Tissue
Anti- CYP1A1 antibody, ASA-B0544,IHC(P)
IHC(P): Rat Kidney Tissue
IHC(P): Rat Kidney Tissue