More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| peptidylprolyl isomerase B(cyclophilin B) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cyclophilin B(176-196aa MEVVRKVESTKTDSRDKPLKD), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PPIB |
Protein | Peptidyl-prolyl cis-trans isomerase B |
Uniprot ID | P23284 |
Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. |
Tissue Specificity | |
Sub-cellular localization | Endoplasmic reticulum lumen. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Sequence Similarities | Belongs to the cyclophilin-type PPIase family. PPIase B subfamily. |
Aliases | AA408962 antibody|AA553318 antibody|AI844835 antibody|Cphn 2 antibody|Cphn2 antibody|Cyclophilin B antibody|Cyclophilin like protein antibody|CyP 20b antibody|CYP S1 antibody|CYP-S1 antibody|CYPB antibody|EC 5.2.1.8 antibody|MGC14109 antibody|MGC2224 antibody|OI9 antibody|peptidyl prolyl cis trans isomerase B antibody|Peptidyl prolyl cis trans isomerase B precursor antibody|Peptidyl-prolyl cis-trans isomerase B antibody|Peptidyl-prolyl cis-trans isomerase B precursor antibody|peptidylprolyl isomerase B(cyclophilin B) antibody|Peptidylprolyl isomerase B antibody|PPIase antibody|PPIase B antibody|PPIB antibody|PPIB_HUMAN antibody|Rotamase antibody|Rotamase B antibody|S cyclophilin antibody|S-cyclophilin antibody|SCYLP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cyclophilin B antibody, ASA-B0539, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: A431 Cell Lysate
Lane 1: Rat Ovary Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: A431 Cell Lysate
Anti-Cyclophilin B antibody, ASA-B0539, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
