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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cyclin D1 | Monoclonal | IgG | Mouse | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Ascites |
Immunogen | ||||||
| Recombinant human cyclin D1 protein. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCND1 |
Protein | G1/S-specific cyclin-D1 |
Uniprot ID | Human(P24385), Mouse(P25322), Rat(P39948) |
Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the cyclin family. Cyclin D subfamily. |
Aliases | BCL1|U21B31|D11S287|PRAD1|B-CELL LEUKEMIA 1|B-cell CLL/lymphoma 1|G1/S-specific cyclin D1|parathyroid adenomatosis 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 4μg/ml | Human, mouse, rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 8μg/ml | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 8μg/ml | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | Suitable | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
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Anti-Cyclin D1 antibody, ASA-B0530, IHC(F)
IHC(F): Mouse Intestine Tissue
IHC(F): Mouse Intestine Tissue
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Anti-Cyclin D1 antibody, ASA-B0530, IHC(F)
IHC(F): Mouse Spleen Tissue
IHC(F): Mouse Spleen Tissue
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Anti-Cyclin D1 antibody, ASA-B0530, IHC(F)
IHC(F): Rat Intestine Tissue
IHC(F): Rat Intestine Tissue