CX3CL1 Antibody View larger

CX3CL1 Antibody

ASA-B0518

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

chemokine(C-X3-C motif) ligand 1 Polyclonal IgG Rabbit Mouse, Rat IHC-P, IHC-F, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1(74-92aa CADPKEKWVQDAMKHLDHQ), identical to the related rat sequence.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

CX3CL1

Protein

Fractalkine

Uniprot ID

O35188

Function

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity). Binds to CX3CR1.

Tissue Specificity

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Sub-cellular localization

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Aliases

A 152E5.2 antibody|AB030188 antibody|ABCD 3 antibody|ABCD3 antibody|AI848747 antibody|C-X3-C motif chemokine 1 antibody|C3Xkine antibody|Chemokine(C-X3-C motif) ligand 1 antibody|Chemokine C X3 C motif ligand 1 antibody|Chemokine CX3C Motif Ligand 1 antibody|CX3C membrane anchored chemokine antibody|CX3C membrane-anchored chemokine antibody|CX3CL1 antibody|CXC 3 antibody|CXC3 antibody|CXC3C antibody|D8Bwg0439e antibody|FKN antibody|Fractalkine antibody|Neurotactin antibody|NTN antibody|NTT antibody|Processed fractalkine antibody|SCYD 1 antibody|SCYD1 antibody|Small inducible cytokine D1 antibody|Small inducible cytokine subfamily D(Cys X3 Cys) member 1 antibody|small inducible cytokine subfamily D(Cys-X3-Cys), member 1(fractalkine, neurotactin) antibody|Small inducible cytokine subfamily D member 1 antibody|Small-inducible cytokine D1 antibody|X3CL1_HUMAN antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Mouse, Rat RatAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Mouse, Rat RatAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CX3CL1 antibody, ASA-B0518, Western blotting
Lane 1: Recombinant Mouse Fractalkin Protein 10ng
Lane 2: Recombinant Mouse Fractalkin Protein 5ng
Lane 3: Recombinant Mouse Fractalkin Protein 2.5ng
Anti-CX3CL1 antibody, ASA-B0518, IHC(P)
IHC(P): Rat Intestine Tissue

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