More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| CUE domain containing 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2(267-287aa EAEEMKATYINLKPARKYRFH), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CUEDC2 |
Protein | CUE domain-containing protein 2 |
Uniprot ID | Q9H467 |
Function | Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism. |
Tissue Specificity | Significantly up-regulated in breast tumor tissues compared with matched adjacent normal tissues (at protein level). Levels inversely correlate with ESR1 in breast cancers and are lower in low-grade tumors compared to high-grade tumors. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the CUEDC2 family. |
Aliases | bA18I14.5 antibody|C10orf66 antibody|Chromosome 10 open reading frame 66 antibody|CUE domain containing 2 antibody|CUE domain-containing protein 2 antibody|CUED2 antibody|CUED2_HUMAN antibody|Cuedc2 antibody|MGC2491 antibody|OTTHUMP00000020368 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CUEDC2 antibody, ASA-B0511, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human CuEDC2, 28.9KD(162aa tag+ N197-H287)
Lane 1: Recombinant Human CuEDC2 Protein 10ng
Lane 2: Recombinant Human CuEDC2 Protein 5ng
Lane 3: Recombinant Human CuEDC2 Protein 2.5ng
Lane 4: Recombinant Human CuEDC2 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant human CuEDC2, 28.9KD(162aa tag+ N197-H287)
Lane 1: Recombinant Human CuEDC2 Protein 10ng
Lane 2: Recombinant Human CuEDC2 Protein 5ng
Lane 3: Recombinant Human CuEDC2 Protein 2.5ng
Lane 4: Recombinant Human CuEDC2 Protein 1.25ng
Anti-CUEDC2 antibody, ASA-B0511, IHC(P)
IHC(P): Human Thyroid Cancer Tissue
IHC(P): Human Thyroid Cancer Tissue
