More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| catenin (cadherin-associated protein), alpha 1, 102kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292). Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTNNA1 |
Protein | Catenin alpha-1 |
Uniprot ID | P35221 |
Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. |
Tissue Specificity | Expressed ubiquitously in normal tissues. |
Sub-cellular localization | Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. |
Sequence Similarities | Belongs to the vinculin/alpha-catenin family. |
Aliases | alpha catenin antibody|alpha E catenin antibody|Alpha E-catenin antibody|alphaE catenin antibody|Cadherin associated protein 102kDa antibody|Cadherin associated protein antibody|Cadherin-associated protein antibody|CAP 102 antibody|CAP102 antibody|Catenin (cadherin associated protein) alpha 1 102kDa antibody|Catenin (cadherin associated protein), alpha 1, 102kDa antibody|Catenin alpha 1 antibody|Catenin alpha-1 antibody|CTNA1_HUMAN antibody|CTNNA 1 antibody|CTNNA1 antibody|FLJ36832 antibody|FLJ52416 antibody|NY REN 13 antigen antibody|OTTHUMP00000224141 antibody|OTTHUMP00000224147 antibody|Renal carcinoma antigen NY REN 13 antibody|Renal carcinoma antigen NY-REN-13 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CTNNA1 antibody, ASA-B0510--1.jpg
All lanes: Anti CTNNA1 (ASA-B0510) at 0.5ug/ml
WB: Human Recombinant CTNNA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti CTNNA1 (ASA-B0510) at 0.5ug/ml
WB: Human Recombinant CTNNA1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-CTNNA1 antibody, ASA-B0510--2.jpg
All lanes: Anti CTNNA1 (ASA-B0510) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 4: NIH3T3 Whole Cell Lysate at 40ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: HEPA Whole Cell Lysate at 40ug
All lanes: Anti CTNNA1 (ASA-B0510) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 4: NIH3T3 Whole Cell Lysate at 40ug
Lane 5: PC-12 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: HEPA Whole Cell Lysate at 40ug
Anti-CTNNA1 antibody, ASA-B0510--3.jpg
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue