More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| connective tissue growth factor | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTGF |
Protein | Connective tissue growth factor |
Uniprot ID | P29279 |
Function | Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. |
Tissue Specificity | Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix . Secreted . |
Sequence Similarities | Belongs to the CCN family. |
Aliases | CCN 2 antibody|CCN family member 2 antibody|CCN2 antibody|Connective Tissue Growth Factor antibody|CTGF antibody|CTGF_HUMAN antibody|Hcs 24 antibody|Hcs24 antibody|Hypertrophic chondrocyte specific protein 24 antibody| Hypertrophic chondrocyte-specific gene product 24 antibody|Hypertrophic chondrocyte-specific protein 24 antibody|IBP-8 antibody|IGF-binding protein 8 antibody|IGFBP-8 antibody|IGFBP8 antibody|Insulin like Growth Factor Binding Protein 8 antibody|Insulin-like Growth Factor-Binding Protein 8 antibody| MGC102839 antibody|NOV 2 antibody|NOV2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CTGF antibody, ASA-B0509, Western blotting
All lanes: Anti CTGF (ASA-B0509) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti CTGF (ASA-B0509) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- CTGF antibody, ASA-B0509, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
