More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| C-terminal binding protein 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CTBP2(429-445aa PNQPTKHGDNREHPNEQ), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTBP2 |
Protein | C-terminal-binding protein 2(CtBP2) |
Uniprot ID | P56545 |
Function | Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity). |
Tissue Specificity | Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. |
Sub-cellular localization | Nucleus . Cell junction, synapse . |
Sequence Similarities | |
Aliases | C terminal binding protein 2 antibody|C-terminal-binding protein 2 antibody|CtBP2 antibody|CTBP2_HUMAN antibody|ribeye antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CTBP2 antibody, ASA-B0506, Western blotting
All lanes: Anti CTBP2 (ASA-B0506) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: U87 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti CTBP2 (ASA-B0506) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: U87 Whole Cell Lysate at 40ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
Anti-CTBP2 antibody, ASA-B0506, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-CTBP2 antibody, ASA-B0506, ICC
ICC: HELA Cell
ICC: HELA Cell