More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| C-terminal binding protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1 (409-440aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CTBP1 |
Protein | C-terminal-binding protein 1 |
Uniprot ID | Q13363 |
Function | Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. |
Tissue Specificity | Expressed in germinal center B-cells. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | BARS antibody|brefeldin A- ribosylated substrate antibody|C terminal binding protein 1 antibody|C-terminal-binding protein 1 antibody|CTBP antibody|CtBP1 antibody|Ctbp1 antibody| CTBP1_HUMAN antibody|MGC104684 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CTBP1 antibody, ASA-B0505, Western blotting
All lanes: Anti CTBP1 (ASA-B0505) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
All lanes: Anti CTBP1 (ASA-B0505) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
Anti- CTBP1 antibody, ASA-B0505, IHC(P)
IHC(P): Mouse Thymus Tissue
IHC(P): Mouse Thymus Tissue
Anti- CTBP1 antibody, ASA-B0505, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue