More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| c-src tyrosine kinase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CSK recombinant protein (Position: S2-G204). Human CSK shares 99% amino acid (aa) sequence identity with both mouse and rat CSK. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CSK |
Protein | Tyrosine-protein kinase CSK |
Uniprot ID | P41240 |
Function | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. |
Tissue Specificity | Expressed in lung and macrophages. |
Sub-cellular localization | Cytoplasm . Cell membrane . Note: Mainly cytoplasmic, also present in lipid rafts. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. |
Aliases | C SRC antibody|C SRC kinase antibody|C src Tyrosine Kinase antibody|C-SRC kinase antibody|c-src tyrosine kinase antibody|Csk A antibody|CSK antibody|CSK_HUMAN antibody|CYTOPLASMIC TYROSINE KINASE antibody|EC 2.7.10.2 antibody|MGC112926 antibody|MGC117393 antibody|MGC154049 antibody|P60 Src antibody|Protein tyrosine kinase CYL antibody|Protein-tyrosine kinase CYL antibody|Proto oncogene tyrosine protein kinase antibody|Tyrosine protein kinase CSK antibody|Tyrosine protein kinase CSK antibody|Tyrosine-protein kinase CSK antibody|zgc:154049 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CSK antibody, ASA-B0503--1.jpg
All lanes: Anti CSK (ASA-B0503) at 0.5ug/ml
WB: Recombinant Human CSK Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti CSK (ASA-B0503) at 0.5ug/ml
WB: Recombinant Human CSK Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-CSK antibody, ASA-B0503--2.jpg
All lanes: Anti CSK (ASA-B0503) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: NIH3T3 Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
Pred
All lanes: Anti CSK (ASA-B0503) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: NIH3T3 Whole Cell Lysate at 40ug
Lane 9: NEURO Whole Cell Lysate at 40ug
Pred
