More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-crk avian sarcoma virus CT10 oncogene homolog | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Crk p38 recombinant protein (Position: A2-R246). Human Crk p38 shares 99% and 100% amino acid (aa) sequences identity with mouse and rat Crk p38, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CRK |
Protein | Adapter molecule crk |
Uniprot ID | P46108 |
Function | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm . Cell membrane . Note: Translocated to the plasma membrane upon cell adhesion. |
Sequence Similarities | Belongs to the CRK family. |
Aliases | Adapter molecule crk antibody|Avian sarcoma virus CT10 (v crk) oncogene homolog antibody|CRK antibody|CRK isoform 2 antibody|CRK isoform II antibody|CRK_HUMAN antibody|CRKII antibody|FLJ38130 antibody|OTTHUMP00000115366 antibody|OTTHUMP00000198330 antibody|p38 antibody|Proto oncogene C crk antibody|Proto-oncogene C-crk antibody|v crk avian sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog (avian) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Crk p38 antibody, ASA-B0499--1.jpg
All lanes: Anti Crk p38 (ASA-B0499) at 0.5ug/ml
WB: Recombinant Human CRKp38 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti Crk p38 (ASA-B0499) at 0.5ug/ml
WB: Recombinant Human CRKp38 Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-Crk p38 antibody, ASA-B0499--2.jpg
All lanes: Anti Crk p38 (ASA-B0499) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
All lanes: Anti Crk p38 (ASA-B0499) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Anti-Crk p38 antibody, ASA-B0499--3.jpg
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue