Cpn10 Antibody View larger

Cpn10 Antibody

ASA-B0495

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

heat shock 10kDa protein 1(chaperonin 10) Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, ICC, WB Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

HSPE1

Protein

10 kDa heat shock protein, mitochondrial(Hsp10)

Uniprot ID

P61604

Function

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

Tissue Specificity

Sub-cellular localization

Mitochondrion matrix.

Sequence Similarities

Aliases

10 kDa chaperonin antibody|10 kDa heat shock protein mitochondrial antibody|10 kDa heat shock protein, mitochondrial antibody|CH10_HUMAN antibody|Chaperonin 10 antibody|Cpn 10 antibody|CPN10 antibody|Early pregnancy factor antibody|Early-pregnancy factor antibody|EPF antibody|GROES antibody|Heat shock 10kD protein 1 antibody|Heat shock 10kD protein 1 chaperonin 10 antibody|Heat shock 10kDa protein 1 antibody|Heat shock 10kDa protein 1 chaperonin 10 antibody|HSP 10 antibody|HSP10 antibody|HSPE 1 antibody|HSPE1 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human MouseAssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cpn10 antibody, ASA-B0495, ICC
ICC: A549 Cell
Anti-Cpn10 antibody, ASA-B0495, Western blotting
All lanes: Anti Cpn10 (ASA-B0495) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Testis Tissue Lysate at 50ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
Pre
Anti-Cpn10 antibody, ASA-B0495, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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