More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase) | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human COX2(589-604aa DDINPTVLLKERSTEL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | PTGS2 |
Protein | Prostaglandin G/H synthase 2 |
Uniprot ID | P35354 |
Function | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. |
Tissue Specificity | |
Sub-cellular localization | Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. |
Sequence Similarities | Belongs to the prostaglandin G/H synthase family. |
Aliases | COX 2 antibody|COX-2 antibody|COX2 antibody|Cyclooxygenase 2 antibody|Cyclooxygenase 2b antibody|Cyclooxygenase antibody|Cyclooxygenase-2 antibody|Cyclooxygenase2 antibody|EC 1.14.99.1 antibody|fj02a10 antibody|Glucocorticoid-regulated inflammatory cyclooxygenase antibody|Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody|GRIPGHS antibody|hCox 2 antibody|Macrophage activation-associated marker protein P71/73 antibody|OTTHUMP00000033524 antibody|PES-2 antibody|PGG/HS antibody|PGH synthase 2 antibody|PGH2_HUMAN antibody|PGHS 2 antibody|PGHS-2 antibody|PGHS2 antibody|PHS 2 antibody|PHS II antibody|PHS2 antibody|Prostaglandin endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase) antibody|Prostaglandin endoperoxide synthase 2 antibody|Prostaglandin G/H synthase 2 antibody|Prostaglandin G/H synthase 2 precursor antibody|Prostaglandin G/H synthase and cyclooxygenase antibody|Prostaglandin G/H synthase antibody|Prostaglandin H2 synthase 2 antibody|prostaglandin-endoperoxide synthase 2(prostaglandin G/H synthase and cyclooxygenase) antibody|Prostaglandin-endoperoxide synthase 2 antibody|PTGS2 antibody|ptgs2a antibody|TIS10 antibody|TIS10 protein antibody|unp1239 antibody|wu:fj02a10 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-COX2/Cyclooxygenase 2 antibody, ASA-B0492, Western blotting
All lanes: Anti COX2/Cyclooxygenase 2 (ASA-B0492) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
All lanes: Anti COX2/Cyclooxygenase 2 (ASA-B0492) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
Anti-COX2/Cyclooxygenase 2 antibody, ASA-B0492, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
